cell viability detection ccg 1423  (TargetMol)

Journal: Redox Biology

Article Title: Myo1f regulates monocyte adhesion and contributes to atherosclerosis via MRTFA-dependent ITGB2 expression

doi: 10.1016/j.redox.2026.104049

Figure Lengend Snippet: Myo1f promotes ITGB2 transcription through MRTFA. A, The adhesion of THP-1 cells to HUVECs after treatment with MRTFA knockdown lentivirus was observed under a fluorescence microscope (scale bar, 50 μm). Adherent cell numbers were calculated from 3 biological replicate images, and data are shown as fold change (ratio to shCtrl). B, Western blot analysis of MRTFA protein levels in THP-1 cells administered or not administered oxLDL (n = 3). C, Confirm the effectiveness of MRTFA knockdown lentivirus by western blotting (n = 3). D, Representative Western blot and quantification of ITGB2 expression after THP-1 knockdown of MRTFA with or without exposure to oxLDL (n = 3). E, Detection of Itgb2 mRNA levels of THP-1 under oxLDL stimulation by qPCR after elimination or not elimination of MRTFA (n = 5). F, Western blotting analysis of ITGB2 expression after gradient increase of MRTFA inhibitor CCG-1423 under oxLDL stimulation (n = 3). G, Representative confocal images of ITGB2 (red) with or without MRTFA knockdown and THP-1 stimulation with oxLDL (scale bar, 20 μm; n = 3). H, Western blot analysis of ITGB2 expression in oxLDL-stimulated THP-1 cells after overexpression of Myo1f with or without elimination of MRTFA (n = 3). I, Nuclear translocation of MRTFA following elimination of Myo1f based on oxLDL stimulation by dissociating cytoplasmic and nuclear analysis (n = 3). Data were presented as the mean ± SEM. Student's t-test (unpaired, two-tailed) for B, E and G . One-way ANOVA followed by Tukey's multiple comparisons test for A, C, D, F, H and I . Each P value is displayed in the image.

Article Snippet: Eight-week-old Apoe −/− mice were randomly divided into groups and simultaneously intraperitoneally injected with the MRTFA inhibitor (CCG-1423, 1 mg/kg, HY-13991, MCE) or vehicle and were given high-cholesterol feed for 8 weeks.

Techniques: Knockdown, Fluorescence, Microscopy, Western Blot, Expressing, Over Expression, Translocation Assay, Two Tailed Test

Journal: Redox Biology

Article Title: Myo1f regulates monocyte adhesion and contributes to atherosclerosis via MRTFA-dependent ITGB2 expression

doi: 10.1016/j.redox.2026.104049

Figure Lengend Snippet: Cytoskeletal polymerization promotes ITGB2 transcription and MRTFA nuclear translocation. A, Western blot analysis of ITGB2 expression after increasing gradient of G-actin polymerization inhibitor LAT-A under oxLDL stimulation (n = 3). B, The adhesion of THP-1 cells to HUVECs after treatment with LAT-A (2 μM) was observed under a fluorescence microscope (scale bar, 50 μm). Adherent cell numbers were calculated from 3 biological replicate images, and data are shown as fold change (ratio to DMSO). C, After overexpressing Myo1f and treating THP-1 cells with or without LAT-A, Western blot analysis was performed on ITGB2 expression under oxLDL stimulation (n = 3). D, Nuclear translocation of MRTFA after LAT-A treatment based on oxLDL stimulation by dissociated cytoplasmic and nuclear analysis (n = 3). E, Representative colocalization immunofluorescence micrographs of phalloidin-labeled F-actin (red) and Myo1f (green) staining in THP-1 cells. The scan line graph represents the F-actin and Myo1f staining intensity along the white straight line (scale bar, 20 μm; n = 3). F, By isolating F-actin and G-actin in THP-1 cells exposed to oxLDL with or without depletion of Myo1f, and quantifying their ratios by immunoblotting (n = 3). G, Effects of knockdown of Myo1f on MRTFA and actin binding in THP-1 cells. MRTFA antibody was used to co-immunoprecipitate actin in THP-1 cells with or without Myo1f knockdown treatment and stimulation with oxLDL (n = 3). Data were presented as the mean ± SEM. Student's t-test (unpaired, two-tailed) for F and G . One-way ANOVA followed by Tukey's multiple comparisons test for A-D . Each P value is displayed in the image.

Article Snippet: Eight-week-old Apoe −/− mice were randomly divided into groups and simultaneously intraperitoneally injected with the MRTFA inhibitor (CCG-1423, 1 mg/kg, HY-13991, MCE) or vehicle and were given high-cholesterol feed for 8 weeks.

Techniques: Translocation Assay, Western Blot, Expressing, Fluorescence, Microscopy, Immunofluorescence, Labeling, Staining, Knockdown, Binding Assay, Two Tailed Test

Journal: Redox Biology

Article Title: Myo1f regulates monocyte adhesion and contributes to atherosclerosis via MRTFA-dependent ITGB2 expression

doi: 10.1016/j.redox.2026.104049

Figure Lengend Snippet: Inhibiting MRTFA alleviates atherosclerosis. A, Schematic diagram of the intervention process by intraperitoneally injecting the MRTFA inhibitor CCG-1423 into Apoe −/− mice. B, Representative images of en face Oil Red O-stained aortas of Apoe −/− and Apoe −/− mice intraperitoneally injected with CCG-1423 are shown (n = 7). C, Representative images of HE and Oil Red O staining of cross-sections of aortic roots in mice with the indicated groups (scale bar, 200 μm; n = 7). D-F, Body weight and total plasma cholesterol and LDL-c levels in specific groups of mice (n = 7). G-I, The serum ALT, AST and Cr levels in specific groups of mice (n = 7). J, Western blot analysis of ITGB2 levels in mouse aortic plaques of mice in indicated groups (n = 7). K, Proposed mechanism of Myo1f in regulating monocyte adhesion and atherosclerosis progression. Data were presented as the mean ± SEM. Student's t-test (unpaired, two-tailed) for B-J . Each P value is displayed in the image.

Article Snippet: Eight-week-old Apoe −/− mice were randomly divided into groups and simultaneously intraperitoneally injected with the MRTFA inhibitor (CCG-1423, 1 mg/kg, HY-13991, MCE) or vehicle and were given high-cholesterol feed for 8 weeks.

Techniques: Staining, Injection, Clinical Proteomics, Western Blot, Two Tailed Test